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KMID : 0545120100200071152
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 7 p.1152 ~ p.1155
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Establishment of an axenic culture of microcystin-producing Microcystis aeruginosa isolated from a Korean reservoir
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Han Ah-Won
Oh Kyoung-Hee
Jheong Weon-Hwa
Cho Young-Cheol
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Abstract
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In order to establish an axenic (bacteria-free) culture of Microcystis aeruginosa NIER 10039 isolated from a Korean reservoir, the culture was subjected to sequential treatment, including ultrasonication, washing, and addition of antibiotics. Three broad-spectrum antibiotics, namely, kanamycin, ampicillin, and imipenem, were applied separately in that order. Axenicity of the culture was confirmed by cultivation on bacterial media and observation under epifluorescence and scanning electron microscopes. We are the first to establish an axenic culture of a Microcystis strain isolated from Korean reservoirs and can be used in physiological and molecular studies to control toxic Microcystis blooms.
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KEYWORD
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axenic culture, Microcystis, Korean reservoir, sequential treatment, antibiotics
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